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Atomic Force Microscopy

AFM_instrument

Axiovert Bioscope II

The AFM produces high resolution images on the order of fractions of a nanometer, which is more than 1000 times higher than the optical limit. Advantages of AFM over electron microscopy include the production of a three dimensional image and minimal sample preparation. A laser beam is deflected off the tip onto a photodiode, which measures the specific mechanical movements of the probe to produce the image of biological or material samples such as proteins, blood cells, bacteria, viruses, polymers, metals, and minerals.