Zeiss Axio Observer 7 Inverted Microscope with Apotome .2 is an inverted microscope with transmitted (brightfield/phase/DIC) and fluorescence illumination. This unit has a motorized Z-axis for optical sectioning. The Apotome attachment allows for structured illumination, which eliminates out of focus/scatted light. Fluorescence filters include DAPI, GFP, and TexasRed. Digital cameras= 6 MP Axiocam 506 monochrome and 5 MP Axiocam 105 color.
Please use this guide or the Thermofisher Fluorescence SpectraViewer to determine which fluorophores will work with our filter set.
| Filter cubes | Matching fluorophores | Avoid using these fluorophores |
| DAPI | Hoechst | Pacific Blue |
| GFP | FITC, GFP, AlexaFluor 488 | AlexaFluor 514, YFP |
| DsRed | Texas Red, tdTomato, AlexaFluor 555 & 594 | Cy3, Cy5, Sytox Red, AlexaFluor 647 |
| Mag | Class | Immersion | Contrast | NA | Cover glass | WD (mm) |
| 5x* | A Plan | Dry | None | 0.12 | Insensitive | 10.10 |
| 10x | EC Plan-Neofluar | Dry | Ph1 | 0.30 | Insensitive | 5.20 |
| 20x | Plan-Apochromat | Dry | DIC | 0.80 | 0.17 mm (#1.5) | 0.55 |
| 40x | Plan-Neofluar | Oil | None | 1.30 | 0.17 mm (#1.5) | 0.21 |
| 63x | Plan-Apochromat | Oil | DIC | 1.40 | 0.17 mm (#1.5) | 0.19 |
| 100x | α-Plan Apochromat | Oil | DIC | 1.46 | 0.17 mm (#1.5) | 0.11 |
* WD = Working distance, which determines the depth of focus
* Please request 5x objective if need, it floats between this microscope and the confocal in the ILSB.
