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Keith R. Porter Imaging Facility

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Keith R. Porter Imaging Facility

  • Instruments
    • Physics Building: NanoImaging Facility
      • Scanning Electron Microscopy
      • Atomic Force Microscopy
      • Sputter/carbon coaters
    • Interdisciplinary Life Sciences Building
      • Cell Sorter/Flow Cytometer
      • Confocal Microscopy
      • Stereomicroscope
      • Image Processing & Analysis
    • Biological Sciences Building
      • Widefield Microscopy
      • Stereomicroscopy
      • Confocal Microscopy
      • Transmission Electron Microscopy
      • Specimen Preparation Equipment
      • 3D Printing
  • Usage Fees and Billing
    • Rates
  • Policy & Scheduling
    • Scheduling Calendars
    • Full Service Imaging
      • Work Request Forms
  • User Resources
    • Instrument SOPs & Training videos
    • Instrument webinars & videos
    • Image Processing Resources
      • Processing optical microscopy files
      • Fiji (ImageJ)
  • About Us
    • Keith Roberts Porter
    • Director
    • Facility-Related Publications
    • Visit the KPIF
  • User Resources
    • Instrument SOPs & Training videos
    • Instrument webinars & videos
    • Image Processing Resources
      • Processing optical microscopy files
      • Fiji (ImageJ)

Fiji (ImageJ)

What is ImageJ? What is Fiji?

ImageJ is a free, java-based image processing software created by NIH developers. It has the ability to add plugins that can expand its capabilities. Fiji (which stands for Fiji is just ImageJ) is bundled with the most commonly used analytical plugins and those needed to open microscope file types (e.g. .lif, .czi).

Getting Started

Measuring Objects

Z-Stack Processing

Determining Fluorescent Intensity and Signal

 

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