Confocal Microscopy

Zeiss LSM 900 confocal, ILSB 324G

The ZEISS LSM 900 Confocal with Airyscan 2 is a super-resolution confocal laser scanning microscope. The inverted Axio Observer 7 microscope includes a motorized stage that allows tiling, capture of multiple data points during time-lapse experiments and a built-in environmental chamber with heat, CO2 and O2 control.

Diode Lasers:
  • 405, 488, 561, 640 nm
Detectors:
  • 2 GaAsP PMT detectors
  • 1 Airyscan detector (for 63×)
  • Transmitted light PMT

 

Magnification Objective Class Immersion Contrast NA Cover glass thickness Working distance (mm)
5x* A Plan Dry None 0.12 Insensitive 10.10
10x Plan-Apochromat Dry DIC 0.45 0.17 mm/ (#1.5) 2.10
20x Plan-Apochromat Dry DIC 0.80 0.17 mm/ (#1.5) 0.55
40x Plan-Apochromat Water/Glycerine (Immersol W/G) DIC *not currently available 1.20 0.15-0.19 mm, correction collar 0.41
63x Plan-Apochromat Oil (Immersol 518) DIC 1.40 0.17 mm/ (#1.5) 0.19

* The 5x objective “floats” between this microscope and the Axio Observer in Biology. Please contact the staff if you need it on the confocal.