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Light Microscopy

Comparison at 20x magnification

  • KPIF Poster created with confocal microscopy

    Leica M205 FA stereomicroscope has motorized controls and magnification of 8-320x. It is equipped with a 5 megapixel Amscope CMOS color digital camera.

  • Zeiss Axio Observer 7 is an inverted fluorescence microscope with the ability to take optical sections. The Apotome attachment allows for structured illumination, which eliminates out of focus/scatted light. Equipped with filters for: DIC, DAPI, GFP, DsRed and a 6 megapixel Axiocam 506 mono digital camera. Objectives: 10X/0.3, 20X/0.8 and 63X/1.4 Oil
  • The Leica SP5 laser scanning confocal microscope can be used for high resolution imaging of a variety of biological and material samples.  The instrument provides high resolution 2D and 3D images. Objectives: 10X/0.4, 20X/0.7 (multi-immersion), 40X/ 1.25 Oil, and 63X/1.4 Oil, 63X/1.2 Water and 100X/1.4 Oil. The system is equipped with the following excitation wavelengths: 405nm, 458nm, 476 nm, 488nm, 496nm, 514nm, 561nm, 594 nm and 633nm. Emission detection ranges from 461-767 nm. Advanced imaging techniques including FCS, FRET and FRAP are available on this instrument.