Confocal Microscopy

The Leica SP5 confocal inverted microscope includes a motorized stage for tiling and capture of multiple data points during one time-lapse experiment. The instrument is equipped with FRET and FRAP software and ISS fluorescence correlation spectroscopy (FCS) hardware that includes two avalanche photodiode detectors (APDs) and a dedicated computer with ISS Vista FCS acquisition and analysis software.

Red algae imaged with Leica SP5 confocal (credit: T. deCarvalho)
Objectives:
  • 10x/0.4 NA
  • 20X/0.7 NA, multi-immersion
  • 40X/1.25 NA, oil, DIC
  • 63x/1.4 NA, oil
  • 63X/1.2 NA, water (for FCS)
  • 100X/1.4 NA, oil
Lasers:
  • UV 405 nm
  • Argon: 458, 476, 488, 496 & 514 nm
  • DPSS 561 nm
  • HeNe 594 nm
  • HeNe 633 nm
Detectors:
  • 4 PMTS: 461-767 nm collection windows
  • 1 transmitted light PMT