The Keith Porter Imaging Facility in room 324 of the Interdisciplinary Life Sciences building houses the following instrumentation:
ZEISS LSM 900 confocal
The ZEISS LSM 900 Confocal with Airyscan 2 is a super-resolution confocal laser scanning microscope. The inverted Axio Observer 7 microscope includes a motorized stage that allows tiling, capture of multiple data points during time-lapse experiments and a built-in environmental chamber with heat, CO2 and O2 control. More information on the configuration and specifications.
BD FACSMelody cell sorter
The BD FACSMelody is capable of detecting up to 10 parameters and 8 colors simultaneously. Samples can be sorted at single cell-basis with sample agitation and temperature control into two or four tubes (5mL round bottom tube to 1.0mL eppendorf), multi-well plates or microscope slides. More information on the configuration and specifications.
ZEISS Axio Zoom
The ZEISS Axio Zoom microscope.V16 is fluorescence on-axis zoom microscope with magnification from 7x-112x. It is equipped with a monochromatic Axiocam 506 camera. Filters include DAPI, GFP and DsRed.
Image processing and analysis computer
The Keith Porter Imaging Facility offers state-of-the-art image processing and analysis capabilities including a custom-built workstation with Imaris 9.5.1 (Oxford) for 3D and 4D analysis, Huygens Essential (SVI) deconvolution software and PerkinElmer Living Image software for IVIS image analysis, as well as assistance with Fiji/ImageJ. Use of the image analysis software is provided free of charge.